One?Step All?Aqueous Interfacial Assembly of Robust Membranes for Long?Term Encapsulation and Culture of Adherent Stem/Stromal Cells

The therapeutic effectiveness and biological relevance of technologies based on adherent cells depend platforms that enable long-term culture in controlled environments. Liquid-core capsules have been suggested as semipermeable moieties with spatial homogeneity due to the high mobility all components their core. lack cell-adhesive sites liquid-core structures often hampers use for stem cell-based survival cell-directed self-organization. Here, one-step fast formation robust polymeric formed by interfacial complexation oppositely charged polyelectrolytes an all-aqueous environment, compatible simultaneous encapsulation mesenchymal stem/stromal (MSCs) microcarriers, is described. adhesion umbilical cord MSCs microcarriers enables aggregation more than 21 days prepared either manually dropwise addition, or scalable electrohydrodynamic atomization, generating stable capsules. Cell secretion overtime can be tailored providing static dynamic (bioreactor)